TẠP CHÍ KHOA HỌC YERSIN
YERSIN JOURNAL OF SCIENCE
ISSN: 2525 - 2372
TẠP CHÍ KHOA HỌC YERSIN
YERSIN JOURNAL OF SCIENCE
Bệnh tim mạch được Tổ chức Y tế Thế giới nhận định là một trong những nguyên nhân gây tử vong hàng đầu hiện nay nếu không được phát hiện và điều trị kịp thời. Flavoids chứa trong các thực phẩm hằng ngày có liên quan chặt chẽ đến sự điều hoà huyết áp của cơ thể và giảm các nguy cơ về tim mạch. Trong nghiên cứu này, (-)-Epicatechin – một dạng flavonoid trong trà xanh được khảo sát tác dụng gây độc cho tim thông qua mô hình stress do H2O2 trong khi các đặc tính bảo vệ tim được chứng minh bằng thử nghiệm MTT và lactate dehydrogenase trên tế bào cơ tim đã biệt hóa H9c2 của chuột. Kết quả cho thấy, sau 48 giờ (-)-Epicatechin có thể gây tổn thương tế bào từ 10 µM. Tuy nhiên, khi điều trị trước với 10, 30 hoặc 100 µM (-)-Epicatechin trong 24 giờ có thể bảo vệ các tế bào H9c2 đã biệt hóa chống lại sự chết tế bào do H2O2.
Cardiovascular disease is considered by the World Health Organization as a silent killer, which is the leading cause of death globally if it is not detected and treated promptly. Flavonoids contained in daily foods are closely related to the regulation of body blood pressure and the reduction of cardiovascular risks. In this paper, flavonoid (-)-epicatechin extracted from green tea was investigated for the cardiotoxic effects, through H2O2-induced oxidative stress modeling, while cardioprotective properties were demonstrated by MTT and lactate dehydrogenase assays on rat differentiated H9c2 cardio myoblasts with various time prolonged. Results show that after 48 hours (-)-Epicatechin can cause cell damage from 10 µM. However, pre-treatment with 10, 30, or 100 µM (-)-Epicatechin in 24 hours could protect differentiated H9c2 cells from H2O2-induced cell death.
flavonoids, catechin, (-)-Epicatechin, bảo vệ tim mạch, độc tính trên tim tế bào H9c2, extracellular signal-regulated kinases, con đường tín hiệu protein kinase ERK1/2.
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