TẠP CHÍ KHOA HỌC YERSIN
YERSIN JOURNAL OF SCIENCE
ISSN: 2525 - 2372
TẠP CHÍ KHOA HỌC YERSIN
YERSIN JOURNAL OF SCIENCE
Đề tài nhằm nghiên cứu khả năng hòa tan lân hữu cơ của 225 dòng Pseudomonas stutzeri được phân lập từ các ao nuôi tôm và cá tra ở 8 tỉnh Đồng Bằng Sông Cửu long (ĐBSCL), trong đó có 52 dòng hòa tan được lân hữu cơ và dòng có khả năng sản sinh phytase cao nhất là N1a. Điều kiện tối ưu cho vi khuẩn Pseudomonas stutzeri N1a sinh enzyme phytase cao nhất là môi trường bán rắn với cơ chất bột bắp ở pH 7.5, nhiệt độ 30 - 40oC, thời gian nuôi cấy là 3 ngày. Bổ sung glucose và sucrose với hàm lượng mỗi chất 0,5% làm tăng sản lượng phytase. Malt extract 1% là nguồn nitơ tối ưu cho sinh tổng hợp phytase của Pseudomonas stutzeri. Khảo sát ảnh hưởng của pH và nhiệt độ lên hoạt tính của phytase thô thu nhận được từ sinh khối vi khuẩn tươi cho thấy nhiệt độ tối ưu cho hoạt động của enzyme phytase là 450C và pH tối ưu là 5.5. Enzyme phytase khá bền ở nhiệt độ -4oC và ổn đinh hoạt tính trong dung dịch pH 5.5 trong 24 giờ.
This study aimed at studying the ability to solubilize organic phosporous of 225 Pseudomonas stutzeri bacteria strains, which were collected from shrimp and catfish ponds in 8 provinces of the Mekong Delta. Among 225 isolated bacteria, 52 were found to be able to solubilize organic phosporous, and Pseudomonas stutzeri N1a bacterium has the highest ability to produce phytase. The optimal condition for Pseudomonas stutzeri N1a to produce the highest phytase enzyme was the semi-solid medium with the cornstarch substrate at pH 7.5 and the temperature of 30 - 40oC after 3 days of incubation. The addition of glucose and sucrose with a concentration of 0,5% each increased the phytase yield. Malt extract of 1% was also a good nitrogen source for phytase biosynthesis. Investigating the effects of pH and temperature on the activity of crude phytase, obtained from Pseudomonas stutzeri biomass, showed that the optimal temperature and pH for phytase enzyme activity were 45oC and 5.5, respectively. This enzyme phytase was stable in the solution at a pH of 5.5 at -4oC within 24 hours.
Enzyme phytase, môi trường bán rắn, nhiệt độ tối ưu, Pseudomonas stutzeri, pH tối ưu.
Enzyme phytase, semi-solid medium, optimal temperature, Pseudomonas stutzeri, optimal pH.
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